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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
18/08/1999 |
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Data da última atualização: |
20/02/2023 |
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Autoria: |
CARVALHO, C. H. S. |
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Afiliação: |
CARLOS H. S. CARVALHO, EMBRAPA-CNPMS. |
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Título: |
Agrobacterium-mediated transformation of sorghum and analysis of putative transposable element-induced mutants in sorghum. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
1999. |
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Páginas: |
99 p. |
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Idioma: |
Inglês |
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Notas: |
Tese (Doutorado) - Purdue University, West Lafayette. |
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Conteúdo: |
Plant biotechnology has become a powerful tool to complement the traditional methods of plants improvement. Several methodologies have been developed to identify and clone agronomically important genes to transfer genes from any living organism to plants. This work encompasses the study of two methodologies related to the identification and transferring of genes in sorghum. The first part reports the development of a protocol for sorghum transformation via Agrobacterium tumefaciens. It is demonstrated that Agrobacterium-mediated transformation is a feasible technique for the genetic transformation of sorghum. Sorghum transgenic plants were produced via Agrobacterium tumefaciens, and the transformation evidenced by Southern blot analysis of To and T1 plants, detection of GUS activity, and production of T1 plants resistant to hygromycin. Immature embryos of sorghum were very sensitive to Agrobacterium, and embryo death after co-cultivation was considered the limiting step to increase the transformation efficiency. Key factors were the co-cultivation medium, the use of a genotype and an explant with good tissue culture response, and the addition of Pluronic F-68 to the inoculation medium. Sorghum transformation via Agrobacterium is still not a routine technique, but it seems to have good potential once the protocol is further refined and improved. The second part of the thesis describes the analysis of several putative transposable element-induced mutants in sorghum identified in a Candystripe population, and the identification of endogenous transposable elements in sorghum derived from the cs1 family. Lastly, a likely involvement of a transposable element in the susceptibility of Colby to Periconia circinata is dicussed. MenosPlant biotechnology has become a powerful tool to complement the traditional methods of plants improvement. Several methodologies have been developed to identify and clone agronomically important genes to transfer genes from any living organism to plants. This work encompasses the study of two methodologies related to the identification and transferring of genes in sorghum. The first part reports the development of a protocol for sorghum transformation via Agrobacterium tumefaciens. It is demonstrated that Agrobacterium-mediated transformation is a feasible technique for the genetic transformation of sorghum. Sorghum transgenic plants were produced via Agrobacterium tumefaciens, and the transformation evidenced by Southern blot analysis of To and T1 plants, detection of GUS activity, and production of T1 plants resistant to hygromycin. Immature embryos of sorghum were very sensitive to Agrobacterium, and embryo death after co-cultivation was considered the limiting step to increase the transformation efficiency. Key factors were the co-cultivation medium, the use of a genotype and an explant with good tissue culture response, and the addition of Pluronic F-68 to the inoculation medium. Sorghum transformation via Agrobacterium is still not a routine technique, but it seems to have good potential once the protocol is further refined and improved. The second part of the thesis describes the analysis of several putative transposable element-induced mutants in sorghum identified ... Mostrar Tudo |
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Thesagro: |
Agrobacterium Tumefaciens; Biotecnologia; Sorghum Bicolor; Sorgo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02330nam a2200181 a 4500
001 1482512
005 2023-02-20
008 1999 bl uuuu m 00u1 u #d
100 1 $aCARVALHO, C. H. S.
245 $aAgrobacterium-mediated transformation of sorghum and analysis of putative transposable element-induced mutants in sorghum.$h[electronic resource]
260 $a1999.$c1999
300 $a99 p.
500 $aTese (Doutorado) - Purdue University, West Lafayette.
520 $aPlant biotechnology has become a powerful tool to complement the traditional methods of plants improvement. Several methodologies have been developed to identify and clone agronomically important genes to transfer genes from any living organism to plants. This work encompasses the study of two methodologies related to the identification and transferring of genes in sorghum. The first part reports the development of a protocol for sorghum transformation via Agrobacterium tumefaciens. It is demonstrated that Agrobacterium-mediated transformation is a feasible technique for the genetic transformation of sorghum. Sorghum transgenic plants were produced via Agrobacterium tumefaciens, and the transformation evidenced by Southern blot analysis of To and T1 plants, detection of GUS activity, and production of T1 plants resistant to hygromycin. Immature embryos of sorghum were very sensitive to Agrobacterium, and embryo death after co-cultivation was considered the limiting step to increase the transformation efficiency. Key factors were the co-cultivation medium, the use of a genotype and an explant with good tissue culture response, and the addition of Pluronic F-68 to the inoculation medium. Sorghum transformation via Agrobacterium is still not a routine technique, but it seems to have good potential once the protocol is further refined and improved. The second part of the thesis describes the analysis of several putative transposable element-induced mutants in sorghum identified in a Candystripe population, and the identification of endogenous transposable elements in sorghum derived from the cs1 family. Lastly, a likely involvement of a transposable element in the susceptibility of Colby to Periconia circinata is dicussed.
650 $aAgrobacterium Tumefaciens
650 $aBiotecnologia
650 $aSorghum Bicolor
650 $aSorgo
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
19/09/2006 |
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Data da última atualização: |
28/05/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SAWAZAKI, E.; GALLO, P. B.; CASTRO, J. L.; SAWAZAKI, H. E.; DUARTE, A.; VIANA, J. M. S.; MIRANDA, G. V.; PACHECO, C. A. P. |
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Afiliação: |
CLESO ANTONIO PATTO PACHECO, CNPMS. |
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Título: |
Comportamento de genótipos de milho pipoca na região Centro-Sul do Estado de São Paulo. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte, Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS, 2006. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Caracteres agronômicos. |
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Thesagro: |
Hibrido; Milho Pipoca; Variedade. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/50220/1/Comportamento-genotipos.pdf
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Marc: |
LEADER 00951nam a2200241 a 4500
001 1475864
005 2018-05-28
008 2006 bl uuuu u00u1 u #d
100 1 $aSAWAZAKI, E.
245 $aComportamento de genótipos de milho pipoca na região Centro-Sul do Estado de São Paulo.$h[electronic resource]
260 $aIn: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte, Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS$c2006
300 $c1 CD-ROM.
650 $aHibrido
650 $aMilho Pipoca
650 $aVariedade
653 $aCaracteres agronômicos
700 1 $aGALLO, P. B.
700 1 $aCASTRO, J. L.
700 1 $aSAWAZAKI, H. E.
700 1 $aDUARTE, A.
700 1 $aVIANA, J. M. S.
700 1 $aMIRANDA, G. V.
700 1 $aPACHECO, C. A. P.
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Embrapa Milho e Sorgo (CNPMS) |
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